CDC37 Polyklonaler Antikörper

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CDC37 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10218-1-PBS

Synonyme

p50Cdc37, Hsp90 co-chaperone Cdc37, N-terminally processed, Hsp90 co-chaperone Cdc37, Hsp90 co chaperone Cdc37, Hsp90 chaperone protein kinase-targeting subunit

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  WB analysis of HEK-293 using 10218-1-AP (same clone as 10218-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 10218-1-AP (same clone as 10218-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  WB analysis of COS-7 using 10218-1-AP (same clone as 10218-1-PBS)

  COS-7 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 10218-1-AP (same clone as 10218-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10218-1-AP (same clone as 10218-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 10218-1-AP (same clone as 10218-1-PBS)

  IP result of anti-CDC37 (IP:10218-1-AP, 4ug; Detection:10218-1-AP 1:1000) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10218-1-AP (same clone as 10218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10218-1-AP (CDC37 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10218-1-AP (same clone as 10218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10218-1-AP (CDC37 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10218-1-AP (same clone as 10218-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CDC37 antibody (10218-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 10218-1-AP (same clone as 10218-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using CDC37 antibody 10218-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 10218-1-AP (same clone as 10218-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 10218-1-AP (Cdc37 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 10218-1-AP (same clone as 10218-1-PBS)

  Immunofluorescent analysis of A431 cells using 10218-1-AP (Cdc37 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 10218-1-AP (same clone as 10218-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug CDC37 Polyclonal antibody (10218-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10218-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA CDC37 und zeigt Reaktivität mit Affe, human, Maus

• Getestete Reaktivität: Affe, human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDC37 fusion protein Ag0267
• Vollständiger Name: cell division cycle 37 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC000083
• Gene symbol: CDC37
• Gene ID (NCBI): 11140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cdc (cell division control) family of proteins are regulatory proteins involved in the processes of cell cycle and proliferation. CDC37, migrated as a protein of 50 kDa in SDS PAGE, is a co-chaperone protein that recruits several immature client kinases to Hsp90 for proper folding. It functions in association with Hsp90, although little is known of its mechanism of action or where it fits into a folding pathway involving other Hsp90 cochaperones. CDC37 up-regulation is a common early event in some localized human cancers. But, the effect of CDC37 dysregulation in human cells is unclear.

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