CDC37 Monoklonaler Antikörper

CDC37 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1C2A6

Kat-Nr. : 66420-1-Ig

Synonyme

CDC37, CDC37A, Hsp90 co chaperone Cdc37, P50CDC37

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Galerie der Validierungsdaten

• 

  WB analysis using 66420-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66420-1-Ig (CDC37 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 66420-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66420-1-Ig(CDC37 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen, ROS1728-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:5000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

66420-1-Ig bindet in WB, IF, ELISA CDC37 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CDC37 fusion protein Ag0267
• Vollständiger Name: cell division cycle 37 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC000083
• Gene symbol: CDC37
• Gene ID (NCBI): 11140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDC(cell division control) family of proteins are regulatory proteins involved in the processes of cell cycle and proliferation. CDC37, migrated as a protein of 50 kDa in SDS PAGE, is a co-chaperone protein that recruits several immature client kinases to Hsp90 for proper folding. It functions in association with Hsp90, although little is known of its mechanism of action or where it fits into a folding pathway involving other Hsp90 cochaperones. CDC37 up-regulation is a common early event in some localized human cancers. But, the effect of CDC37 dysregulation in human cells is unclear.

Publikationen

Species	Application	Title
human	IF	Acta Pharmacol Sin; Okicamelliaside targets the N-terminal chaperone pocket of HSP90 disrupts the chaperone protein interaction of HSP90-CDC37 and exerts antitumor activity.; Authors - Chuan-Jing Cheng