CDC37L1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CDC37L1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 16293-1-AP

Synonyme

CDC37B, CDC37L1, HARC

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Galerie der Validierungsdaten

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  WB analysis using 16293-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16293-1-AP (CDC37L1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 16293-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16293-1-AP (CDC37L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16293-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16293-1-AP (CDC37L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16293-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16293-1-AP (CDC37L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16293-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16293-1-AP (CDC37L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16293-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16293-1-AP (CDC37L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16293-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16293-1-AP (CDC37L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Nierengewebe, Jurkat-Zellen, LNCaP-Zellen, MCF-7-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

16293-1-AP bindet in WB, IHC, ELISA CDC37L1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDC37L1 fusion protein Ag9459
• Vollständiger Name: cell division cycle 37 homolog (S. cerevisiae)-like 1
• Berechnetes Molekulargewicht: 337 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC014133
• Gene symbol: CDC37L1
• Gene ID (NCBI): 55664
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB, IHC	J Cancer; CDC37L1 acts as a suppressor of migration and proliferation in gastric cancer by down-regulating CDK6.; Authors - Li Li ; KD Validated
human	WB,IHC	Cell Death Discov; miR-15a and miR-20b sensitize hepatocellular carcinoma cells to sorafenib through repressing CDC37L1 and consequent PPIA downregulation.; Authors - Li Li