CDC40 Rekombinanter Antikörper

CDC40 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242034D1

Kat-Nr. : 84679-4-RR

Synonyme

242034D1, Cell division cycle 40 homolog, EH binding protein 3, EHB3, hPRP17

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  WB analysis using 84679-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84679-4-RR (CDC40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of K-562 using 84679-4-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CDC40 antibody (84679-4-RR, Clone: 242034D1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84679-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84679-4-RR against Human CDC40 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, HUVEC-Zellen, Jurkat-Zellen, K-562-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IF/ICC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84679-4-RR bindet in WB, IF/ICC, ELISA CDC40 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CDC40 fusion protein Ag36151
• Vollständiger Name: cell division cycle 40 homolog (S. cerevisiae)
• Beobachtetes Molekulargewicht: 65-67 kDa
• GenBank-Zugangsnummer: BC126114
• Gene symbol: CDC40
• Gene ID (NCBI): 51362
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDC40 (cell division cycle 40), also known as EHB3. It is expected to be located in the nucleus, which is ubiquitinated in bone marrow and lymph node. The protein is required for pre-mRNA splicing as component of the activated spliceosome, and it plays an important role in embryonic brain development; this function does not require proline isomerization (PMID: 33220177; 33220177). The molecular weight of CDC40 is 65 kDa.