CDC42EP4 Polyklonaler Antikörper

CDC42EP4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19262-1-AP

Synonyme

CEP4, Cdc42 effector protein 4, BORG4, Binder of Rho GTPases 4

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  WB analysis of K-562 using 19262-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19262-1-AP (CDC42EP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 19262-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19262-1-AP (CDC42EP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 19262-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19262-1-AP (CDC42EP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 19262-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 19262-1-AP (CDC42EP4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 19262-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 19262-1-AP (CDC42EP4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, A549-Zellen, Mauslungengewebe
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19262-1-AP bindet in WB, IHC, ELISA CDC42EP4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDC42EP4 fusion protein Ag13594
• Vollständiger Name: CDC42 effector protein (Rho GTPase binding) 4
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 43-45 kDa
• GenBank-Zugangsnummer: BC010451
• Gene symbol: CDC42EP4
• Gene ID (NCBI): 23580
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDC42EP4, or Cell division cycle 42 effector protein 4, is a member of the CDC42-binding protein family. This protein interacts with Rho family GTPases and plays a role in regulating the organization of the actin cytoskeleton. It has been shown to bind both CDC42 and TC10 GTPases in a GTP-dependent manner. Overexpression of CDC42EP4 in fibroblasts can induce pseudopodia formation, suggesting its involvement in actin filament assembly and cell shape control. CDC42EP4 is also implicated in cancer progression, particularly in prostate cancer. Furthermore, CDC42EP4 has been implicated in the regulation of glutamate transporters, which are crucial for maintaining glutamate homeostasis in the nervous system. It has been suggested that CDC42EP4 may interact with septins, a family of polymerizing GTPases that constitute the membrane skeleton, and this interaction could potentially influence the localization and activity of glutamate transporters.