CDC5L Rekombinanter Antikörper

CDC5L Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250089G5

Kat-Nr. : 85788-5-RR

Synonyme

CDC5, Cdc5-like protein, CEF1, Cell division cycle 5-like protein, KIAA0432

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  WB analysis using 85788-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85788-5-RR (CDC5L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85788-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC5L antibody (85788-5-RR, Clone: 250089G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85788-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85788-5-RR against Human CDC5L were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HCT 116-Zellen, HepG2-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:8000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85788-5-RR bindet in WB, IF/ICC, ELISA CDC5L und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CDC5L fusion protein Ag3578
• Vollständiger Name: CDC5 cell division cycle 5-like (S. pombe)
• Berechnetes Molekulargewicht: 802 aa, 92 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC001568
• Gene symbol: CDC5L
• Gene ID (NCBI): 988
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDC5L is ubiquitously expressed in humans, with relatively high expression levels in testis, bone marrow, and 25 other tissues. In proliferating cells, CDC5L is constitutively expressed, suggesting its regulation may occur through post-translational modifications. CDC5L plays a critical role in the cell cycle, particularly in the G2/M transition. It interacts with cyclins and cyclin-dependent kinases (CDKs), helping to control the timing and accuracy of cell division.