Cdc7 Kinase Polyklonaler Antikörper

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• KD/KO Validated

Cdc7 Kinase Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 17980-1-AP

Synonyme

EC:2.7.11.1, Cell division cycle 7-related protein kinase, CDC7-related kinase, CDC7L1, CDC7 related kinase

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  WB analysis of SH-SY5Y using 17980-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17980-1-AP (Cdc7 Kinase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17980-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17980-1-AP (Cdc7 Kinase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 17980-1-AP

  Immunofluorescent analysis of HepG2 cells, using CDC7 antibody 17980-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

17980-1-AP bindet in WB, IF/ICC, ELISA Cdc7 Kinase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cdc7 Kinase fusion protein Ag12384
• Vollständiger Name: cell division cycle 7 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 574 aa, 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC111044
• Gene symbol: CDC7
• Gene ID (NCBI): 8317
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; EGFR-ERK induced activation of GRHL1 promotes cell cycle progression by up-regulating cell cycle related genes in lung cancer.; Authors - Yiming He
human	WB	Cell Death Discov; Identifying CDC7 as a synergistic target of chemotherapy in resistant small-cell lung cancer via CRISPR/Cas9 screening; Authors - Ling Deng ; KD Validated
	WB	BMC Urol; Clinical significance and pro-oncogenic function of DBF4 in clear cell renal cell carcinoma; Authors - Liuyan Chen