CDCA2 Polyklonaler Antikörper

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• KD/KO Validated

CDCA2 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17701-1-PBS

Synonyme

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  WB analysis of Jurkat using 17701-1-AP (same clone as 17701-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17701-1-AP (CDCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17701-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 17701-1-AP (same clone as 17701-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17701-1-AP (CDCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17701-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 17701-1-AP (same clone as 17701-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17701-1-AP (CDCA2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17701-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 17701-1-AP (same clone as 17701-1-PBS)

  IP result of anti-CDCA2 (IP:17701-1-AP, 4ug; Detection:17701-1-AP 1:800) with Jurkat cells lysate 2800ug. This data was developed using the same antibody clone with 17701-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 17701-1-AP (same clone as 17701-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17701-1-AP (CDCA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17701-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17701-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA CDCA2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDCA2 fusion protein Ag11967
• Vollständiger Name: cell division cycle associated 2
• Berechnetes Molekulargewicht: 113 kDa
• Beobachtetes Molekulargewicht: 125 kDa
• GenBank-Zugangsnummer: BC036214
• Gene symbol: CDCA2
• Gene ID (NCBI): 157313
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cell division cycle associated 2(CDCA2) is a nuclear protein that is a specific regulatory subunit for protein phosphatase 1γ (PP1γ). CDCA2 is a candidate factor involved in preparing mitotic chromatin for the transition to interphase, and controls PP1γ-dependent essential DDR regulationCDCA2 regulates the DNA damage response(DDR) in cell cycle. In addition, CDCA2 modulates phosphorylation of major mitotic histone H3 in a PP1-dependent manner. We got 125 kDa in western blotting maybe due to its phosphorylation.