CDCA3 Polyklonaler Antikörper

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• KD/KO Validated

CDCA3 Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 15594-1-AP

Synonyme

TOME 1, GRCC8, Gene-rich cluster protein C8, Gene rich cluster protein C8, Cell division cycle-associated protein 3

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  IHC staining of human breast cancer using 15594-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15594-1-AP (CDCA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15594-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15594-1-AP (CDCA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15594-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15594-1-AP (CDCA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 15594-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CDCA3 antibody (15594-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 7 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

15594-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA CDCA3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDCA3 fusion protein Ag7951
• Vollständiger Name: cell division cycle associated 3
• Berechnetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC002551
• Gene symbol: CDCA3
• Gene ID (NCBI): 83461
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDCA3, also named C8, GRCC8, and TOME1, is an F-box-like protein required for entry into mitosis. It acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at the G2/M phase.

Publikationen

Species	Application	Title
human	WB	Oncogene; CPSF4 regulates circRNA formation and microRNA mediated gene silencing in hepatocellular carcinoma.; Authors - Xueying Wang
human	IHC	Cancer Cell Int; The inhibitory effect of silencing CDCA3 on migration and proliferation in bladder urothelial carcinoma.; Authors - Dexin Shen ; KD Validated
human	WB,IHC	Naunyn Schmiedebergs Arch Pharmacol; Curcumin targets miR-134-5p to suppress the progression of colorectal cancer through regulating the CDCA3/CDK1 pathway; Authors - Fu Liu
human	WB,IHC,IF,CoIP	Biochem Biophys Res Commun; CDCA3 promotes cell proliferation by activating the NF-κB/cyclin D1 signaling pathway in colorectal cancer.; Authors - Wei Zhang ; KD Validated
human	WB,IHC	Pathol Res Pract; miR-145-5p suppresses proliferation, metastasis and EMT of colorectal cancer by targeting CDCA3.; Authors - Qing Chen
human	WB,IF	J Oncol; CDCA3 Predicts Poor Prognosis and Affects CD8+ T Cell Infiltration in Renal Cell Carcinoma; Authors - Yuanyuan Bai