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CDCA4 Polyklonaler Antikörper

CDCA4 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11625-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of HeLa cells using CDCA4 Polyclonal antibody (11625-1-AP)

    WB analysis of HeLa using 11625-1-AP (same clone as 11625-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11625-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CDCA4 Polyclonal antibody (11625-1-AP)

    WB analysis using 11625-1-AP (same clone as 11625-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11625-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CDCA4 Polyclonal antibody (11625-1-AP)

    WB analysis of HeLa using 11625-1-AP (same clone as 11625-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11625-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CDCA4 Polyclonal antibody (11625-1-AP)

    WB analysis of HEK-293 using 11625-1-AP (same clone as 11625-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11625-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

11625-1-PBS bindet in WB, Indirect ELISA CDCA4 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CDCA4 fusion protein Ag2214
Vollständiger Name cell division cycle associated 4
Berechnetes Molekulargewicht 241 aa, 26 kDa
Beobachtetes Molekulargewicht 33 kDa
GenBank-ZugangsnummerBC025263
Gene symbol CDCA4
Gene ID (NCBI) 55038
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIP-Br proteins are a set of proteins with the potential to function in both transcriptional control and cell cycle regulation [PMID:18572021]. CECA4 is a member of TRIP-br family, based on its homologous sequence motifs including SERTA and a PHD-bromo-binding site which interacts with a series of bromodomain containing transcriptional cofactors[PMID:17141982]. When activated by E2F, CDCA4 consequently functions as a suppressor of E2F-responsive promoters to participate the regulation of cell proliferation through the E2F/pRb pathway [PMID:16098148].