CDCA7 Polyklonaler Antikörper

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• KD/KO Validated

CDCA7 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Rezensionen (2)

Kat-Nr. : 15249-1-AP

Synonyme

Protein JPO1, JPO1, Cell division cycle-associated protein 7

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• 

  WB analysis using 15249-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15249-1-AP (CDCA7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15249-1-AP

  WB result of CDCA7 antibody (15249-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDCA7 transfected HeLa cells.

• 

  IP experiment of HEK-293 using 15249-1-AP

  IP result of anti-CDCA7 (IP:15249-1-AP, 4ug; Detection:15249-1-AP 1:2000) with HEK-293 cells lysate 1480 ug.

• 

  IHC staining of human colon cancer using 15249-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 15249-1-AP (CDCA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15249-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 15249-1-AP (CDCA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15249-1-AP

  Immunofluorescent analysis of HepG2 cells using 15249-1-AP (CDCA7 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, Raji-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

15249-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CDCA7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDCA7 fusion protein Ag4297
• Vollständiger Name: cell division cycle associated 7
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 40-43 kDa
• GenBank-Zugangsnummer: BC027966
• Gene symbol: CDCA7
• Gene ID (NCBI): 83879
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDCA7, also named as JP01, participates in MYC-mediated cell transformation and apoptosis. It plays a role as transcriptional regulator.

Publikationen

Species	Application	Title
mouse	WB,IF	Nucleic Acids Res; The C-terminal 4CXXC-type zinc finger domain of CDCA7 recognizes hemimethylated DNA and modulates activities of chromatin remodeling enzyme HELLS; Authors - Akeo Shinkai
mouse	WB	Sci Adv; CDCA7-associated global aberrant DNA hypomethylation translates to localized, tissue-specific transcriptional responses; Authors - Maja Vukic
human	WB	Proc Natl Acad Sci U S A; HELLS and CDCA7 comprise a bipartite nucleosome remodeling complex defective in ICF syndrome.; Authors - Christopher Jenness
human	WB	J Exp Med; Loss of ZBTB24 impairs nonhomologous end-joining and class-switch recombination in patients with ICF syndrome.; Authors - Angela Helfricht
		Hum Mol Genet; Converging disease genes in ICF syndrome: ZBTB24 controls expression of CDCA7 in mammals.; Authors - Haoyu Wu
human	WB	Bioorg Chem; Novel isatin-based hybrids as potential anti-rheumatoid arthritis drug candidates: Synthesis and biological evaluation; Authors - Jian Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

XIAOBING (Verified Customer) (11-05-2019)

CDCA7 antibody was used to stain the 293T cells transfected with human CDCA7 plasmid. Overexpressed CDCA7 (Red) was localized in the nucleus (DAPI was used to label the nucleus).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: 293T overexpressing human CDCA7

CDCA7 Antibody Immunofluorescence, validation (1:250 dilution) in 293T overexpressing human CDCA7 (Cat no:15249-1-AP)

FH

Guillaume (Verified Customer) (09-08-2019)

I used cells mutated for ZBTB24 which control CDCA7 expression to test the specificity of the antibody. In WB and IP, the signal seem to be lower than the expected size in kDa. This CDCA7 antibody is not very efficient in ChIP

• Applications: Western Blot, Immunoprecipitation, ChIP,
• Cell Tissue Type: Fibroblasts and Lymphoblastoid cells