CDCA7 Polyklonaler Antikörper

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• KD/KO Validated

CDCA7 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15249-1-PBS

Synonyme

Protein JPO1, JPO1, Cell division cycle-associated protein 7

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  WB analysis using 15249-1-AP (same clone as 15249-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 15249-1-AP (CDCA7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15249-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15249-1-AP (same clone as 15249-1-PBS)

  WB result of CDCA7 antibody (15249-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDCA7 transfected HeLa cells. This data was developed using the same antibody clone with 15249-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 15249-1-AP (same clone as 15249-1-PBS)

  IP result of anti-CDCA7 (IP:15249-1-AP, 4ug; Detection:15249-1-AP 1:2000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 15249-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15249-1-AP (same clone as 15249-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 15249-1-AP (CDCA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15249-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15249-1-AP (same clone as 15249-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 15249-1-AP (CDCA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15249-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 15249-1-AP (same clone as 15249-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 15249-1-AP (CDCA7 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 15249-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15249-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CDCA7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDCA7 fusion protein Ag4297
• Vollständiger Name: cell division cycle associated 7
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 40-43 kDa
• GenBank-Zugangsnummer: BC027966
• Gene symbol: CDCA7
• Gene ID (NCBI): 83879
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDCA7, also named as JP01, participates in MYC-mediated cell transformation and apoptosis. It plays a role as transcriptional regulator.