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CDCA8 Monoklonaler Antikörper

CDCA8 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A10C9

Kat-Nr. : 68510-1-PBS

Synonyme

2A10C9, BOR, Borealin, Cell division cycle-associated protein 8, Dasra B

Filter:
  • Western Blot (WB) analysis of various lysates using CDCA8 Monoclonal antibody (68510-1-Ig)

    WB analysis using 68510-1-Ig (same clone as 68510-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68510-1-Ig (CDCA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68510-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CDCA8 Monoclonal antibody (68510-1-Ig)

    IF Staining of A431 using 68510-1-Ig (same clone as 68510-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CDCA8 antibody (68510-1-Ig, Clone: 2A10C9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68510-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CDCA8 Monoclonal antibody (68510-1-Ig)

    IF Staining of A549 using 68510-1-Ig (same clone as 68510-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CDCA8 antibody (68510-1-Ig, Clone: 2A10C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68510-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68510-1-PBS bindet in WB, IF/ICC, Indirect ELISA CDCA8 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen CDCA8 fusion protein Ag3136
Vollständiger Name cell division cycle associated 8
Berechnetes Molekulargewicht 280 aa, 31 kDa
Beobachtetes Molekulargewicht 31-38 kDa
GenBank-ZugangsnummerBC016944
Gene symbol CDCA8
Gene ID (NCBI) 55143
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDCA8, also named as Borealin, Dasra-B and PESCRG3, is a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The predicted molecular weight of CDCA8 is about 31 kDa. This protein can be migrated to 35-40 kDa with phosphor modification or ~50 kDa with SUMO modification.