E-cadherin Polyklonaler Antikörper

E-cadherin Polyklonal Antikörper für WB, IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 20648-1-AP

Synonyme

E-cadherin

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  WB analysis of PC-3 using 20648-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20648-1-AP (E-cadherin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 20648-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 20648-1-AP (E-cadherin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 20648-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20648-1-AP (E-cadherin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 20648-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20648-1-AP (E-cadherin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 20648-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20648-1-AP (E-cadherin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 20648-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20648-1-AP (E-cadherin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 20648-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 20648-1-AP (E-cadherin antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 20648-1-AP

  Immunofluorescent analysis of HepG2 cells, using CDH1 antibody 20648-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 3 publications below

Produktinformation

20648-1-AP bindet in WB, IHC, IF/ICC, ELISA E-cadherin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cadherin 1, type 1, E-cadherin (epithelial)
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 100-125 kDa
• GenBank-Zugangsnummer: NM_004360
• Gene symbol: E-cadherin
• Gene ID (NCBI): 999
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. This antibody is specific to CDH1.

Publikationen

Species	Application	Title
Human	WB	Aging (Albany NY); M6A-related bioinformatics analysis reveals that HNRNPC facilitates progression of OSCC via EMT.; Authors - Guang-Zhao Huang
human	WB	Cancer Lett; SDF-1/CXCR4 Promotes epithelial-mesenchymal transition and progression of colorectal cancer by activation of the wnt/β-catenin signaling pathway.; Authors - Ting-Hua Hu
human	WB	Onco Targets Ther; Bioinformatics Analyses Indicate That Cathepsin G (CTSG) is a Potential Immune-Related Biomarker in Oral Squamous Cell Carcinoma (OSCC).; Authors - Guang-Zhao Huang
human	WB, IF	Endocrine; Adipogenic changes of hepatocytes in a high-fat diet-induced fatty liver mice model and non-alcoholic fatty liver disease patients.; Authors - Xiaoli Pan
mouse	IF	Tissue Cell; Slc44a2 deletion alters tetraspanin and N-cadherin expression: Reduced adhesion and enhanced proliferation in cultured mesenchymal lung cells.; Authors - Thankam S Nair
human	WB	Mol Med Rep; MicroRNA-590 is an EMT-suppressive microRNA involved in the TGFβ signaling pathway.; Authors - Tianming Liu