Cadherin-20 Polyklonaler Antikörper

Cadherin-20 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22049-1-AP

Synonyme

Cadherin 20, cadherin 20, type 2, Cadherin-20, CDH20, Cdh7, CDH7L3

---

Galerie der Validierungsdaten

• 

  WB analysis using 22049-1-AP

  Human placenta tissue were subjected to SDS PAGE followed by western blot with 22049-1-AP (Cadherin-20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22049-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22049-1-AP (Cadherin-20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 22049-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22049-1-AP (Cadherin-20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 22049-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 22049-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 22049-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 22049-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, HeLa-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22049-1-AP bindet in WB, IHC, ELISA Cadherin-20 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cadherin-20 fusion protein Ag16990
• Vollständiger Name: cadherin 20, type 2
• Berechnetes Molekulargewicht: 801 aa, 89 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC101048
• Gene symbol: CDH20
• Gene ID (NCBI): 28316
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.