P-cadherin Polyklonaler Antikörper

P-cadherin Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 13773-1-AP

Synonyme

Cadherin 3, CDH3, CDHP, HJMD, PCAD, P-cadherin

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Galerie der Validierungsdaten

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  WB analysis of A431 using 13773-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13773-1-AP (P-cadherin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 13773-1-AP

  IP Result of anti-P-cadherin (IP:13773-1-AP, 4ug; Detection:13773-1-AP 1:300) with A431 cells lysate 3040ug.

• 

  IHC staining of human breast cancer using 13773-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13773-1-AP (P-cadherin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13773-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13773-1-AP (P-cadherin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13773-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13773-1-AP (P-cadherin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13773-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13773-1-AP (P-cadherin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13773-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13773-1-AP (P-cadherin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 13773-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13773-1-AP (P-cadherin antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of A431 using 13773-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 13773-1-AP (P-cadherin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 13773-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 13773-1-AP (P-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A431 using 13773-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human P-cadherin (13773-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

13773-1-AP bindet in WB, IP, IHC, IF, FC, ELISA P-cadherin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P-cadherin fusion protein Ag4758
• Vollständiger Name: cadherin 3, type 1, P-cadherin (placental)
• Berechnetes Molekulargewicht: 829 aa, 91 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC041846
• Gene symbol: CDH3
• Gene ID (NCBI): 1001
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.

Publikationen

Species	Application	Title
mouse	IF	Nat Commun; Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice.; Authors - Hans I-Chen Harn
mouse	IF	Mol Ther Nucleic Acids; Inhibition of the lncRNA MIAT prevents podocyte injury and mitotic catastrophe in diabetic nephropathy.; Authors - Ziyang Wang
human	IHC	Aging (Albany NY); Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism.; Authors - Jing Han
human	WB,IHC	Front Oncol; Screening and Identification of Key Biomarkers of Gastric Cancer: Three Genes Jointly Predict Gastric Cancer.; Authors - Meng-Jie Shan
human	WB,IHC	Int J Biol Sci; KLF4-Mediated CDH3 Upregulation Suppresses Human Hepatoma Cell Growth and Migration via GSK-3β Signaling.; Authors - Li Li
mouse	IF	Dev Dyn; Denticleless E3 ubiquitin protein ligase (DTL) maintains the proliferation and differentiation of epidermis and hair follicles during skin development; Authors - Yanhui Lin