P-cadherin Monoklonaler Antikörper

P-cadherin Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1G10F7

Kat-Nr. : 66295-1-Ig

Synonyme

Cadherin 3, CDH3, CDHP, HJMD, P cadherin, PCAD, P-cadherin, Placental cadherin

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  WB analysis of A431 using 66295-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66295-1-Ig (P-cadherin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 66295-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66295-1-Ig (P-cadherin antibody) at dilution of 1:250 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 66295-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66295-1-Ig (P-cadherin antibody) at dilution of 1:250 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66295-1-Ig bindet in WB, IHC, ELISA P-cadherin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: P-cadherin fusion protein Ag4758
• Vollständiger Name: cadherin 3, type 1, P-cadherin (placental)
• Berechnetes Molekulargewicht: 829 aa, 91 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC041846
• Gene symbol: P-cadherin
• Gene ID (NCBI): 1001
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.

Publikationen

Species	Application	Title
mouse,human	WB	Biosens Bioelectron; Constructing a highly sensitive duplex immunoassay using AuNPs and AgNPs as nanolabels for investigating the epithelial-mesenchymal transition occurring on circulating tumor cells with lung cancer patients; Authors - Yunuo Dai