CDK1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

CDK1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240937C5

Kat-Nr. : 84271-1-PBS

Synonyme

CDC2, 240937C5, CDC28A, CDKN1, Cell division protein kinase 1

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  WB analysis using 84271-1-RR (same clone as 84271-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84271-1-RR (CDK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84271-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 84271-1-RR (same clone as 84271-1-PBS)

  WB result of CDK1 antibody (84271-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK1 transfected HeLa cells. This data was developed using the same antibody clone with 84271-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84271-1-RR (same clone as 84271-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK1 antibody (84271-1-RR, Clone: 240937C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84271-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84271-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84271-1-RR against Human CDK1 were performed. The affinity constant is 34.3 nM.

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Geprüfte Anwendungen

Produktinformation

84271-1-PBS bindet in WB, IF/ICC, Indirect ELISA CDK1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CDK1 fusion protein Ag29018
• Vollständiger Name: cell division cycle 2, G1 to S and G2 to M
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC014563
• Gene symbol: CDK1
• Gene ID (NCBI): 983
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa. This antibody may have cross reaction with CDK2.