CDK13 Polyklonaler Antikörper

CDK13 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30461-1-PBS

Synonyme

CDC2L5, CHED, CDC2L, CDC2 related protein kinase 5

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  WB analysis using 30461-1-AP (same clone as 30461-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30461-1-AP (CDK13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30461-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 30461-1-AP (same clone as 30461-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30461-1-AP (CDK13 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30461-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 30461-1-AP (same clone as 30461-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK13 antibody (30461-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30461-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 30461-1-AP (same clone as 30461-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CDK13 (30461-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 30461-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30461-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA CDK13 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDK13 fusion protein Ag32897
• Vollständiger Name: cell division cycle 2-like 5 (cholinesterase-related cell division controller)
• Berechnetes Molekulargewicht: 165 kDa
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: NM_003718.4
• Gene symbol: CDK13
• Gene ID (NCBI): 8621
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin-dependent kinase 13 (CDK13) is also named as CDC2L, CDC2L5, CHED and KIAA1791, and belongs to the CMGC Ser/Thr protein kinase family. Interestingly, in TNBC cells silencing CDK13 provokes cell death without affecting DDR genes. Like CDK12, CDK13 phosphorylates the Ser2 position of the heptad repeat in the CTD of RNA Pol II (PMID:26748711). CDK13/CycK complex is that regulates transcription by phosphorylating serine residues at positions 5 and 2 of the heptapeptide repeats (Y-S-P-T-S-P-S) comprising the C-terminal domain (CTD) of RPB1, the largest subunit of RNA polymerase II (RNAPII). CDK13 is expressed in fetal brain, liver, and muscle, and adult whole brain, hippocampus, and bone marrow (PMID:30904094).