CDK2 Rekombinanter Antikörper

CDK2 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240580G11

Kat-Nr. : 83635-2-PBS

Synonyme

240580G11, CDKN2, Cell division protein kinase 2, Cyclin dependent kinase 2, Cyclin-dependent kinase 2

---

• 

  WB analysis using 83635-2-RR (same clone as 83635-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83635-2-RR (CDK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83635-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 83635-2-RR (same clone as 83635-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83635-2-RR (CDK2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83635-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 83635-2-RR (same clone as 83635-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83635-2-RR (CDK2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83635-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83635-2-RR (same clone as 83635-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK2 antibody (83635-2-RR, Clone: 240580G11 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83635-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83635-2-RR (same clone as 83635-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK2 Recombinant antibody (83635-2-RR, Clone:240580G11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83635-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83635-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83635-2-PBS against Human CDK2 was performed. The affinity constant is 4.09 nM.

• 

  Affinity and Kinetic Characterization of 83635-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83635-2-RR against Human CDK2 were performed. The affinity constant is 3.23 nM.

---

Geprüfte Anwendungen

Produktinformation

83635-2-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA CDK2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CDK2 fusion protein Ag17133
• Vollständiger Name: cyclin-dependent kinase 2
• Berechnetes Molekulargewicht: 298 aa, 34 kDa
• Beobachtetes Molekulargewicht: 30-33 kDa
• GenBank-Zugangsnummer: BC003065
• Gene symbol: CDK2
• Gene ID (NCBI): 1017
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.