CDK2 Rekombinanter Antikörper

CDK2 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240580A11

Kat-Nr. : 83635-4-PBS

Synonyme

EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2, CDKN2

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  WB analysis using 83635-4-RR (same clone as 83635-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83635-4-RR (CDK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83635-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83635-4-RR (same clone as 83635-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK2 Recombinant antibody (83635-4-RR, Clone:240580A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83635-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83635-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83635-4-RR against Human CDK2 were performed. The affinity constant is 8.32 nM.

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Geprüfte Anwendungen

Produktinformation

83635-4-PBS bindet in WB, FC (Intra), ELISA CDK2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CDK2 fusion protein Ag17133
• Vollständiger Name: cyclin-dependent kinase 2
• Berechnetes Molekulargewicht: 298 aa, 34 kDa
• Beobachtetes Molekulargewicht: 30-33 kDa
• GenBank-Zugangsnummer: BC003065
• Gene symbol: CDK2
• Gene ID (NCBI): 1017
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.