CDK2AP1 Polyklonaler Antikörper

CDK2AP1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 13060-2-AP

Synonyme

CDK2 associated protein 1, CDK2-associated protein 1, CDKAP1, Cyclin-dependent kinase 2-associated protein 1, Deleted in oral cancer 1

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  WB analysis of HEK-293 using 13060-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13060-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13060-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13060-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 13060-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2AP1 antibody (13060-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Hirngewebe, humanes Herzgewebe, humanes Lebergewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

13060-2-AP bindet in WB, IHC, IF/ICC, ELISA CDK2AP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDK2AP1 fusion protein Ag3736
• Vollständiger Name: cyclin-dependent kinase 2 associated protein 1
• Berechnetes Molekulargewicht: 115 aa, 13 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC034717
• Gene symbol: CDK2AP1
• Gene ID (NCBI): 8099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Oncol Lett; Cathepsin L interacts with CDK2-AP1 as a potential predictor of prognosis in patients with breast cancer.; Authors - Zhan Wang