CDK3 Polyklonaler Antikörper

CDK3 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55103-1-PBS

Synonyme

CDKN3, EC:2.7.11.22, Cyclin-dependent kinase 3, Cyclin dependent kinase 3, Cell division protein kinase 3

---

• 

  WB analysis using 55103-1-AP (same clone as 55103-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 55103-1-AP (same clone as 55103-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 55103-1-AP (same clone as 55103-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 55103-1-AP (same clone as 55103-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55103-1-AP (same clone as 55103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55103-1-AP (CDK3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55103-1-AP (same clone as 55103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55103-1-AP (CDK3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 55103-1-AP (same clone as 55103-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK3 antibody (55103-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 55103-1-AP (same clone as 55103-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK3 Polyclonal antibody (55103-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

55103-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA CDK3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cyclin-dependent kinase 3
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: NM_001258
• Gene symbol: CDK3
• Gene ID (NCBI): 1018
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDK3 belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It is probably involved in the control of the cell cycle. CDK3 interacts with a yet unknown type of cyclin. It can phosphorylate histone H1. Endogenous Cyclin C/Cdk3 complexes are critical to promote exit from quiescence in an Rb-dependent manner. Cyclin C/Cdk3 complexes is a key regulators of cell cycle reentry in human cells. (PMID: 15130482 )The antibody is specific to CDK3.