CDK5 Rekombinanter Antikörper

CDK5 Rekombinant Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241908F10

Kat-Nr. : 84749-1-PBS

Synonyme

241908F10, Cdk 5, Cyclin-dependent kinase 5, Cyclin-dependent-like kinase 5, EC:2.7.11.1

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  WB analysis using 84749-1-RR (same clone as 84749-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84749-1-RR (CDK5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84749-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 84749-1-RR (same clone as 84749-1-PBS)

  IP result of anti-CDK5 (IP:84749-1-RR, 4ug; Detection:84749-1-RR 1:8000) with mouse brain tissue lysate 2120 ug. This data was developed using the same antibody clone with 84749-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 84749-1-RR (same clone as 84749-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84749-1-RR (CDK5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84749-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 84749-1-RR (same clone as 84749-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84749-1-RR (CDK5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84749-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 84749-1-RR (same clone as 84749-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CDK5 antibody (84749-1-RR, Clone: 241908F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84749-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84749-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CDK5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CDK5 fusion protein Ag28465
• Vollständiger Name: cyclin-dependent kinase 5
• Berechnetes Molekulargewicht: 31 kDa, 33 kDa
• Beobachtetes Molekulargewicht: 31-33 kDa
• GenBank-Zugangsnummer: BC005115
• Gene symbol: CDK5
• Gene ID (NCBI): 1020
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.