CDK5R1 Monoklonaler Antikörper

CDK5R1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A3A5

Kat-Nr. : 67503-1-PBS

Synonyme

CDK5 activator 1, CDK5P35, CDK5R, CDK5R1, NCK5A, p23, p25, p35, p35nck5a, TPKII regulatory subunit

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  WB analysis using 67503-1-Ig (same clone as 67503-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse cerebellum using 67503-1-Ig (same clone as 67503-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 67503-1-Ig (same clone as 67503-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67503-1-Ig (same clone as 67503-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67503-1-Ig (same clone as 67503-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67503-1-Ig (same clone as 67503-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67503-1-Ig (CDK5R1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67503-1-Ig (same clone as 67503-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67503-1-Ig (CDK5R1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67503-1-Ig (same clone as 67503-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67503-1-Ig (CDK5R1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67503-1-Ig (same clone as 67503-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67503-1-Ig (CDK5R1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67503-1-PBS bindet in WB, IHC, Indirect ELISA CDK5R1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CDK5R1 fusion protein Ag13152
• Vollständiger Name: cyclin-dependent kinase 5, regulatory subunit 1 (p35)
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC020580
• Gene symbol: CDK5R1
• Gene ID (NCBI): 8851
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin dependent kinase-5 (Cdk5), is an atypical but essential member of the Cdk family of proline-directed serine/threonine kinases. CDK5R1 encodes p35, a specific activator of the serine/threonine kinase CDK5, which plays a central role in neuronal migration and differentiation during central nervous system development. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form.