CDK5RAP3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CDK5RAP3 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11007-1-PBS

Synonyme

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  WB analysis using 11007-1-AP (same clone as 11007-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11007-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 11007-1-AP (same clone as 11007-1-PBS)

  WB result of CDK5RAP3 antibody (11007-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK5RAP3 transfected HEK-293 cells. This data was developed using the same antibody clone with 11007-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 11007-1-AP (same clone as 11007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11007-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 11007-1-AP (same clone as 11007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11007-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11007-1-AP (same clone as 11007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11007-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 11007-1-AP (same clone as 11007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11007-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver using 11007-1-AP (same clone as 11007-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CDK5RAP3 antibody (11007-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11007-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11007-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA CDK5RAP3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDK5RAP3 fusion protein Ag1474
• Vollständiger Name: CDK5 regulatory subunit associated protein 3
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC009957
• Gene symbol: CDK5RAP3
• Gene ID (NCBI): 80279
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin-dependent kinase 5 (CDK5) regulatory subunit associated protein 3 (CDK5RAP3, also named as C53 or LZAP) was initially identified as a binding protein of CDK5 activator p35. CDK5RAP3 is widely expressed in human tissues and broadly located in subcellular compartments. By interacting with other proteins, CDK5RAP3 participates in the regulation of multiple cellular processes including cell cycle, apoptosis, cell invasion, signaling transduction, autophagy, UFMylation and endoplasmic reticulum (ER) stress.