CDK6 Polyklonaler Antikörper

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• KD/KO Validated

CDK6 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Kat-Nr. : 19117-1-AP

Synonyme

CDKN6, Cell division protein kinase 6, Cyclin dependent kinase 6, Cyclin-dependent kinase 6, EC:2.7.11.22

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  WB analysis using 19117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19117-1-AP (CDK6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19117-1-AP (CDK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19117-1-AP

  WB result of CDK6 antibody (19117-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK6 transfected HeLa cells.

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  IP experiment of Jurkat using 19117-1-AP

  IP result of anti-CDK6 (IP:19117-1-AP, 4ug; Detection:19117-1-AP 1:1000) with Jurkat cells lysate 1960 ug.

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  IHC staining of human lung cancer using 19117-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19117-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 19117-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19117-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 19117-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 19117-1-AP (CDK6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 19117-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 19117-1-AP (CDK6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human endometrial cancer using 19117-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19117-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 19117-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19117-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19117-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK6 antibody (19117-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 19117-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK6 (19117-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Endometriumkarzinomgewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 18 publications below
• IHC: See 1 publications below

Produktinformation

19117-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA CDK6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDK6 fusion protein Ag5600
• Vollständiger Name: cyclin-dependent kinase 6
• Berechnetes Molekulargewicht: 326 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36-40 kDa
• GenBank-Zugangsnummer: BC027989
• Gene symbol: CDK6
• Gene ID (NCBI): 1021
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).

Publikationen

Species	Application	Title
human	WB	Theranostics; Estrogen-induced circRNA, circPGR, functions as a ceRNA to promote estrogen receptor-positive breast cancer cell growth by regulating cell cycle-related genes.; Authors - Lei Wang
human	WB	Cancers (Basel); A Patient-Derived Xenograft Model of Dedifferentiated Endometrial Carcinoma: A Proof-of-Concept Study for the Identification of New Molecularly Informed Treatment Approaches.; Authors - Chiao-Yun Lin
human	WB	Oncotarget; High expression of N-myc (and STAT) interactor predicts poor prognosis and promotes tumor growth in human glioblastoma.; Authors - Delong Meng
human	WB	Front Pharmacol; Oxymatrine Inhibits Renal Cell Carcinoma Progression by Suppressing β-Catenin Expression.; Authors - Yinshan Jin
human	WB	Cancer Biother Radiopharm; γ-Glutamylcyclotransferase Knockdown Inhibits Growth of Lung Cancer Cells Through G0/G1 Phase Arrest.; Authors - Zhifeng Lin
human	WB	Cancer Med; δ-Catenin regulates proliferation and apoptosis in renal cell carcinoma via promoting β-catenin nuclear localization and activating its downstream target genes.; Authors - Lincheng Ju