CDK7 Monoklonaler Antikörper

CDK7 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E7B6

Kat-Nr. : 67889-1-PBS

Synonyme

39 kDa protein kinase, CAK, CAK1, CDK activating kinase, CDK7, CDKN7, Cell division protein kinase 7, cyclin dependent kinase 7, MO15, p39 Mo15, p39MO15, STK1

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  WB analysis using 67889-1-Ig (same clone as 67889-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67889-1-Ig (CDK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.

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  WB analysis using 67889-1-Ig (same clone as 67889-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67889-1-Ig (CDK7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67889-1-Ig (same clone as 67889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67889-1-Ig (CDK7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67889-1-Ig (same clone as 67889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67889-1-Ig (CDK7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67889-1-Ig (same clone as 67889-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK7 antibody (67889-1-Ig, Clone: 1E7B6 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67889-1-Ig (same clone as 67889-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CDK7 antibody (67889-1-Ig, Clone: 1E7B6 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67889-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CDK7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CDK7 fusion protein Ag25737
• Vollständiger Name: cyclin-dependent kinase 7
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC005298
• Gene symbol: CDK7
• Gene ID (NCBI): 1022
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.