CDK8 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CDK8 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 22067-1-AP

Synonyme

CDK8, Cell division protein kinase 8, cyclin dependent kinase 8, K35, Mediator complex subunit CDK8, Protein kinase K35

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Galerie der Validierungsdaten

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  WB analysis using 22067-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22067-1-AP (CDK8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22067-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22067-1-AP (CDK8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22067-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22067-1-AP (CDK8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 22067-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 22067-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 22067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 22067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of NIH/3T3 using 22067-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 22067-1-AP (CDK8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of NIH/3T3 using 22067-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human CDK8 (22067-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Mausembryogewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen
• Erfolgreiche Detektion in FC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

22067-1-AP bindet in WB, IHC, IF, FC, ELISA CDK8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDK8 fusion protein Ag17148
• Vollständiger Name: cyclin-dependent kinase 8
• Berechnetes Molekulargewicht: 464 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC069634
• Gene symbol: CDK8
• Gene ID (NCBI): 1024
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; Loss of MED12 activates the TGFβ pathway to promote chemoresistance and replication fork stability in BRCA-deficient cells.; Authors - Lindsey M Jackson ; KD Validated
human	WB	PLoS Genet; Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.; Authors - Emily M Schleicher ; KD Validated
human	WB	Biochim Biophys Acta Gene Regul Mech; PP2A and its adapter protein IER5 induce the DNA-binding ability and target gene expression of E2F1 via dephosphorylation at serine 375; Authors - Hiroto Takeuchi
mouse	WB,IHC	Bull Exp Biol Med; Knockdown of Long Noncoding RNA CCAT2 Suppresses Malignant Phenotype in Human Laryngeal Squamous Cell Carcinoma; Authors - M Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

SITING (Verified Customer) (04-02-2021)

can see band between 50-65kd

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: EMBRYO HEART

CDK8 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:22067-1-AP)