CDK8 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CDK8 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Rezensionen (1)

Kat-Nr. : 22067-1-AP

Synonyme

Cell division protein kinase 8, Cyclin dependent kinase 8, Cyclin-dependent kinase 8, EC:2.7.11.22, K35

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• 

  WB analysis using 22067-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22067-1-AP (CDK8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22067-1-AP

  WB result of CDK8 antibody (22067-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK8 transfected HEK-293 cells.

• 

  WB analysis of HeLa using 22067-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22067-1-AP (CDK8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22067-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22067-1-AP (CDK8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 22067-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 22067-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 22067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 22067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 22067-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 22067-1-AP (CDK8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NIH/3T3 using 22067-1-AP

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug CDK8 Polyclonal antibody (22067-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Mausembryogewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen
• Erfolgreiche Detektion in FC (Intra): NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

22067-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA CDK8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDK8 fusion protein Ag17148
• Vollständiger Name: cyclin-dependent kinase 8
• Berechnetes Molekulargewicht: 464 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC069634
• Gene symbol: CDK8
• Gene ID (NCBI): 1024
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; Loss of MED12 activates the TGFβ pathway to promote chemoresistance and replication fork stability in BRCA-deficient cells.; Authors - Lindsey M Jackson ; KD Validated
human	WB	PLoS Genet; Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.; Authors - Emily M Schleicher ; KD Validated
human	WB	Biochim Biophys Acta Gene Regul Mech; PP2A and its adapter protein IER5 induce the DNA-binding ability and target gene expression of E2F1 via dephosphorylation at serine 375; Authors - Hiroto Takeuchi
mouse	WB,IHC	Bull Exp Biol Med; Knockdown of Long Noncoding RNA CCAT2 Suppresses Malignant Phenotype in Human Laryngeal Squamous Cell Carcinoma; Authors - M Li
mouse,human	WB,IP,IF	J Adv Res; CDK8 mediated inflammatory microenvironment aggravates osteoarthritis progression; Authors - Zhongnan Lin ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

SITING (Verified Customer) (04-02-2021)

can see band between 50-65kd

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: EMBRYO HEART

CDK8 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:22067-1-AP)