CDK8 Rekombinanter Antikörper

CDK8 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240598G9

Kat-Nr. : 83621-6-RR

Synonyme

K35, EC:2.7.11.22, Cyclin-dependent kinase 8, Cyclin dependent kinase 8, Cell division protein kinase 8

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  WB analysis using 83621-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83621-6-RR (CDK8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 83621-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK8 antibody (83621-6-RR, Clone: 240598G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of Caco-2 using 83621-6-RR

  1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug CDK8 Recombinant antibody (83621-6-RR, Clone:240598G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83621-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83621-6-RR against Human CDK8 were performed. The affinity constant is 12.5 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HCT 116-Zellen, HEK-293-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): Caco-2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83621-6-RR bindet in WB, IF/ICC, FC (Intra), ELISA CDK8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CDK8 fusion protein Ag17148
• Vollständiger Name: cyclin-dependent kinase 8
• Berechnetes Molekulargewicht: 464 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC069634
• Gene symbol: CDK8
• Gene ID (NCBI): 1024
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.