CDK9 Rekombinanter Antikörper

CDK9 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240600E3

Kat-Nr. : 83662-2-PBS

Synonyme

240600E3, C 2k, C-2K, CDC2L4, Cell division cycle 2-like protein kinase 4

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  WB analysis using 83662-2-RR (same clone as 83662-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83662-2-RR (CDK9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83662-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00630-4

  Sandwich ELISA standard curve of MP00630-4, Human CDK9 Recombinant Matched Antibody Pair - PBS only. 83662-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2318. 83662-2-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL.

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  Sample test of MP00630-4

  The mean CDK9 concentration was determined to be 5,413.5 pg/mL in HEK-293T cell extract based on a 5.8 mg/mL extract load and 5,349.7 Pg/mL in Jurkat cell extract based on a 1.5 mg/mL extract load.

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  IHC staining of human lung cancer using 83662-2-RR (same clone as 83662-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83662-2-RR (CDK9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83662-2-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 83662-2-RR (same clone as 83662-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83662-2-RR (CDK9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83662-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83662-2-RR (same clone as 83662-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-2-RR, Clone: 240600E3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 83662-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83662-2-RR (same clone as 83662-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK9 Recombinant antibody (83662-2-RR, Clone:240600E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83662-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83662-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83662-2-PBS against Human CDK9 was performed. The affinity constant is 0.145 nM.

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  Affinity and Kinetic Characterization of 83662-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83662-2-RR against Human CDK9 were performed. The affinity constant is 0.305 nM.

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Geprüfte Anwendungen

Produktinformation

83662-2-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test CDK9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CDK9 fusion protein Ag2318
• Vollständiger Name: cyclin-dependent kinase 9
• Berechnetes Molekulargewicht: 372 aa, 43 kDa
• Beobachtetes Molekulargewicht: 38-42 kDa, 55 kDa
• GenBank-Zugangsnummer: BC001968
• Gene symbol: CDK9
• Gene ID (NCBI): 1025
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243). CDK9 has two isoforms with the molecular mass of 42 kDa and 55 kDa, and the relative abundance of Cdk9(42kDa) and Cdk9(55kDa) changes in different cell types (PMID: 12706900, 15780980).