CDK9 Rekombinanter Antikörper

CDK9 Rekombinant Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240600D7

Kat-Nr. : 83662-7-RR

Synonyme

CTK1, Cell division cycle 2-like protein kinase 4, CDC2L4, C-2K, C 2k

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  IHC staining of human lung cancer using 83662-7-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83662-7-RR (CDK9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 83662-7-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83662-7-RR (CDK9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 83662-7-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-7-RR, Clone: 240600D7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 83662-7-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-7-RR, Clone: 240600D7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 83662-7-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-7-RR, Clone: 240600D7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 83662-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83662-7-RR against Human CDK9 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83662-7-RR bindet in IHC, IF/ICC, ELISA CDK9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CDK9 fusion protein Ag2318
• Vollständiger Name: cyclin-dependent kinase 9
• Berechnetes Molekulargewicht: 372 aa, 43 kDa
• Beobachtetes Molekulargewicht: 38-42 kDa, 55 kDa
• GenBank-Zugangsnummer: BC001968
• Gene symbol: CDK9
• Gene ID (NCBI): 1025
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243).