CDKN2AIP Polyklonaler Antikörper

CDKN2AIP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 16615-1-AP

Synonyme

CDKN2A-interacting protein, CARF

---

• 

  WB analysis using 16615-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16615-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16615-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16615-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16615-1-AP

  IP result of anti-CDKN2AIP (IP:16615-1-AP, 4ug; Detection:16615-1-AP 1:500) with HeLa cells lysate 2800ug.

• 

  IHC staining of human kidney using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 16615-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDKN2AIP antibody (16615-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Lebergewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

16615-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CDKN2AIP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDKN2AIP fusion protein Ag9907
• Vollständiger Name: CDKN2A interacting protein
• Berechnetes Molekulargewicht: 579 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC022270
• Gene symbol: CDKN2AIP
• Gene ID (NCBI): 55602
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IHC,IF	Cell Biosci; CDKN2AIP is critical for spermiogenesis and germ cell development; Authors - Yuming Cao
mouse	IF	Acta Biochim Biophys Sin (Shanghai); CARF regulates the alternative splicing and piwi/piRNA complexes during mouse spermatogenesis through PABPC1; Authors - Yuming Cao
human	WB	Clin Cosmet Investig Dermatol; Genome Wide Differential Expression Profiles in Nevus Sebaceous Uncovered Low Expression of CDKN2AIP and Construction of a ceRNA Network.; Authors - Xianhong Yang
mouse	WB,IF	Int J Cardiol; Reduced myocardial CARF and the underlying implications in left ventricular noncompaction cardiomyopathy; Authors - Liu Hui ; KO Validated