CDO1 Polyklonaler Antikörper

CDO1 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 12589-1-AP

Synonyme

CDO, CDO I, CDO1, Cysteine dioxygenase type 1, cysteine dioxygenase, type I

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 12589-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12589-1-AP (CDO1 antibody) at dilution of 1:3000 incubated at 4 degree celsius over night.

• 

  WB analysis of rat liver using 12589-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12589-1-AP (CDO1 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse liver using 12589-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12589-1-AP (CDO1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 12589-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12589-1-AP (CDO1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 12589-1-AP

  IP Result of anti-CDO1 (IP:12589-1-AP, 3ug; Detection:12589-1-AP 1:600) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12589-1-AP (CDO1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12589-1-AP (CDO1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12589-1-AP (CDO1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12589-1-AP (CDO1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12589-1-AP (CDO1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12589-1-AP (CDO1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12589-1-AP (CDO1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12589-1-AP (CDO1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Pankreaskarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 6 publications below

Produktinformation

12589-1-AP bindet in WB, IP, IHC, ELISA CDO1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDO1 fusion protein Ag3294
• Vollständiger Name: cysteine dioxygenase, type I
• Berechnetes Molekulargewicht: 200 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC024241
• Gene symbol: CDO1
• Gene ID (NCBI): 1036
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDO1(cysteine dioxygenase type 1) is also named as CDO and belongs to the cysteine dioxygenase family. It is an enzyme that adds molecular oxygen to the sulfur of cysteine, converting the thiol to a sulfinic acid known as cysteinesulfinic acid (3-sulfinoalanine) and CDO1 is one of the most highly regulated metabolic enzymes responding to diet(PMID:19011731). The expression of CDO can significantly decrease the level of intracellular cysteine and this reduction is also associated with a decrease in total glutathione levels(PMID:17327371). Cysteine dioxygenase (CDO) plays a critical role in the regulation of cellular cysteine concentration andmultiple forms of CDO (23 kDa, 25 kDa, and 68 kDa) have been claimed based upon separation and detection using SDS-PAGE/western blotting(PMID: 14752623).

Publikationen

Species	Application	Title
human	WB,IHC	Cell Death Discov; Cysteine dioxygenase 1 attenuates the proliferation via inducing oxidative stress and integrated stress response in gastric cancer cells; Authors - Gang Ma
human	IHC	Int J Biol Sci; Ferroptosis-associated molecular classification characterized by distinct tumor microenvironment profiles in colorectal cancer.; Authors - Wenqin Luo
mouse	WB	J Nutr Biochem; PMID: 24917045; Authors - Kerstin E Geillinger
rat	WB	J Ethnopharmacol; Integration of metabolomics and transcriptomics to reveal anti-chronic myocardial ischemia mechanism of Gualou Xiebai decoction.; Authors - Fengyun Zhang
human	IHC	Ann Surg Oncol; Promoter DNA Hypermethylation of the Cysteine Dioxygenase 1 (CDO1) Gene in Intraductal Papillary Mucinous Neoplasm (IPMN).; Authors - Yoshiki Fujiyama
mouse	WB	Cell Mol Neurobiol; Taurine Alleviates Chronic Social Defeat Stress-Induced Depression by Protecting Cortical Neurons from Dendritic Spine Loss.; Authors - Yuanyuan Zhu