CDX2 Monoklonaler Antikörper

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• KD/KO Validated

CDX2 Monoklonal Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5A4E6

Kat-Nr. : 60243-1-PBS

Synonyme

5A4E6, Caudal type homeobox 2, Caudal type homeobox protein 2, Caudal-type homeobox protein 2, CDX 3

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  WB analysis of COLO 320 using 60243-1-Ig (same clone as 60243-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60243-1-PBS in a different storage buffer formulation.

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  WB analysis of SW 1990 using 60243-1-Ig (same clone as 60243-1-PBS)

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60243-1-PBS in a different storage buffer formulation.

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  WB analysis of pig colon using 60243-1-Ig (same clone as 60243-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60243-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 60243-1-Ig (same clone as 60243-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60243-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 60243-1-Ig (same clone as 60243-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (60243-1-Ig, Clone: 5A4E6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60243-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50121-1

  Cytometric bead array standard curve of MP50121-1, CDX2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60243-1-PBS. Detection antibody: 60243-3-PBS. Standard:Ag17644. Range: 0.098-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

60243-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA CDX2 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CDX2 fusion protein Ag17644
• Vollständiger Name: caudal type homeobox 2
• Berechnetes Molekulargewicht: 313 aa, 34 kDa
• Beobachtetes Molekulargewicht: 33-40 kDa
• GenBank-Zugangsnummer: BC014461
• Gene symbol: CDX2
• Gene ID (NCBI): 1045
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.