CDX2 Rekombinanter Antikörper

CDX2 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3J1

Kat-Nr. : 82659-1-RR

Synonyme

CDX-2, CDX 3, Caudal-type homeobox protein 2, Caudal type homeobox protein 2, Caudal type homeobox 2

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  WB analysis using 82659-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82659-1-RR (CDX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 82659-1-RR

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 82659-1-RR (CDX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 82659-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82659-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 82659-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82659-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 82659-1-RR

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human rectal cancer using 82659-1-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human rectal cancer using 82659-1-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 82659-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 82659-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Caco-2 using 82659-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (82659-1-RR, Clone: 3J1 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  Affinity and Kinetic Characterization of 82659-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82659-1-RR against Human CDX2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, COLO 320-Zellen, Maus-Kolongewebe
• Erfolgreiche Detektion in IHC: Ratten-Kolongewebe, humanes Kolonkarzinomgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Caco-2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

82659-1-RR bindet in WB, IHC, IF/ICC, ELISA CDX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CDX2 fusion protein Ag17310
• Vollständiger Name: caudal type homeobox 2
• Berechnetes Molekulargewicht: 313 aa, 34 kDa
• Beobachtetes Molekulargewicht: 33-40 kDa
• GenBank-Zugangsnummer: BC014461
• Gene symbol: CDX2
• Gene ID (NCBI): 1045
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.

Publikationen

Species	Application	Title
human	IF	Biofabrication; 3D bioprinting platform development for high-throughput cancer organoid models construction and drug evaluation; Authors - Rui Dai