CDYL Polyklonaler Antikörper

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• KD/KO Validated

CDYL Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 17763-1-AP

Synonyme

CDY like, CDYL, CDYL1, chromodomain protein, Y like, Chromodomain Y like protein

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  WB analysis of PC-3 using 17763-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17763-1-AP (CDYL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17763-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17763-1-AP (CDYL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 17763-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17763-1-AP (CDYL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 17763-1-AP

  IP result of anti-CDYL (IP:17763-1-AP, 4ug; Detection:17763-1-AP 1:1000) with MCF-7 cells lysate 3600 ug.

• 

  IHC staining of human breast cancer using 17763-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17763-1-AP (CDYL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17763-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17763-1-AP (CDYL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 17763-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17763-1-AP (CDYL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 17763-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17763-1-AP (CDYL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, MCF-7-Zellen, Maushodengewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

17763-1-AP bindet in WB, IHC, IF, IP, ELISA CDYL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDYL fusion protein Ag12143
• Vollständiger Name: chromodomain protein, Y-like
• Berechnetes Molekulargewicht: 544 aa, 61 kDa
• Beobachtetes Molekulargewicht: 66 kDa, 60 kDa
• GenBank-Zugangsnummer: BC108725
• Gene symbol: CDYL
• Gene ID (NCBI): 9425
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IHC	Endocrinology; Dynamic profiles and transcriptional preferences of histone modifications during spermiogenesis.; Authors - Yinchuan Li
mouse	WB	Adv Sci (Weinh); Cdyl Deficiency Brakes Neuronal Excitability and Nociception through Promoting Kcnb1 Transcription in Peripheral Sensory Neurons.; Authors - Zhao-Wei Sun
rat	WB	Drug Des Devel Ther; Sorafenib Attenuates Fibrotic Hepatic Injury Through Mediating Lysine Crotonylation; Authors - Xiao-Feng Chen
human	WB	Cancer Lett; CDYL knockdown reduces glioma development through an antitumor immune response in the tumor microenvironment; Authors - Jin-Ge Kong ; KD Validated
human,mouse	WB,IHC,IF	Acta Pharmacol Sin; Chromodomain Y-like (CDYL) inhibition ameliorates acute kidney injury in mice by regulating tubular pyroptosis; Authors - Ting Xiang
human	WB,IF	Cell Rep; The chromodomain protein CDYL confers forebrain identity to human cortical organoids by inhibiting neuronatin; Authors - Yaming Yang ; KO Validated