CEA Polyklonaler Antikörper

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• KD/KO Validated

CEA Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10421-1-AP

Synonyme

Carcinoembryonic antigen, CD66e, CEA, CEACAM5, Meconium antigen 100

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Galerie der Validierungsdaten

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  WB analysis using 10421-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10421-1-AP (CEA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10421-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10421-1-AP (CEA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10421-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10421-1-AP (CEA antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10421-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10421-1-AP (CEA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 10421-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10421-1-AP (CEA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10421-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10421-1-AP (CEA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 10421-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10421-1-AP (CEA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 10421-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10421-1-AP (CEA antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 10421-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10421-1-AP (CEA antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human colon cancer using 10421-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CEA antibody (10421-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human colon cancer using 10421-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CEA antibody (10421-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Appendizitis-Gewebe, humanes Lungenkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Kolonkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• ChIP: See 1 publications below

Produktinformation

10421-1-AP bindet in WB, IHC, IF, chIP, ELISA CEA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEA fusion protein Ag0672
• Vollständiger Name: carcinoembryonic antigen-related cell adhesion molecule 5
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 180-200 kDa, 77 kDa
• GenBank-Zugangsnummer: BC034671
• Gene symbol: CEACAM5
• Gene ID (NCBI): 1048
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.

Publikationen

Species	Application	Title
human	WB	Transl Res; Molecular signature of interleukin-22 in colon carcinoma cells and organoid models.; Authors - Ina Rudloff
human	WB,IHC,chIP	Int J Biol Sci; FBW7 suppresses metastasis of colorectal cancer by inhibiting HIF1α/CEACAM5 functional axis.; Authors - Qingguo Li ; KD Validated
human	IHC	Gene Ther; Lentivirus-mediated RASSF1A expression suppresses aggressive phenotypes of gastric cancer cells in vitro and in vivo.; Authors - P-H Zhou
human	IHC	World Neurosurg; Discovery of Aquaporin-1 and Aquaporin-4 Expression in an Intramedullary Spinal Cord Ependymal Cyst: Case Report.; Authors - Matthew J Shepard
human	WB	PLoS One; Correlation of alpha-1 antitrypsin levels and exosome associated neutrophil elastase endothelial injury in subjects with SARS-CoV2 infection; Authors - Jorge Lascano
mouse	WB	Iran J Basic Med Sci; Preparation and application of patient-derived xenograft mice model of colorectal cancer; Authors - Yutao Zhang

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