CEA/CD66e Monoklonaler Antikörper

CEA/CD66e Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2B8H6

Kat-Nr. : 68377-1-PBS

Synonyme

CEA, 2B8H6, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, CD66e

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  WB analysis using 68377-1-Ig (same clone as 68377-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68377-1-Ig (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68377-1-Ig (same clone as 68377-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68377-1-Ig (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68377-1-Ig (same clone as 68377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEACAM5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68377-1-Ig (same clone as 68377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEACAM5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68377-1-Ig (same clone as 68377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68377-1-Ig (same clone as 68377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 68377-1-Ig (same clone as 68377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 68377-1-Ig (same clone as 68377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 68377-1-Ig (same clone as 68377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 68377-1-Ig (same clone as 68377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68377-1-PBS bindet in WB, IHC, ELISA CEA/CD66e und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CEA/CD66e fusion protein Eg0247
• Vollständiger Name: carcinoembryonic antigen-related cell adhesion molecule 5
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 180-200 kDa
• GenBank-Zugangsnummer: BC034671
• Gene symbol: CEA
• Gene ID (NCBI): 1048
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.