CEA/CD66e Rekombinanter Antikörper

CEA/CD66e Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240666B12

Rezensionen (1)

Kat-Nr. : 83653-5-RR

Synonyme

CEA, CEACAM5, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen

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  WB analysis of MCF-7 using 83653-5-RR

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 83653-5-RR (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human saliva using 83653-5-RR

  human saliva were subjected to SDS PAGE followed by western blot with 83653-5-RR (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 83653-5-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83653-5-RR (CEA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 83653-5-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83653-5-RR (CEA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HT-29 using 83653-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CEA antibody (83653-5-RR, Clone: 240666B12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83653-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83653-5-RR against Human CEA were performed. The affinity constant is 0.734 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, human saliva
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83653-5-RR bindet in WB, IHC, IF/ICC, ELISA CEA/CD66e und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: carcinoembryonic antigen-related cell adhesion molecule 5
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: NM_004363
• Gene symbol: CEA
• Gene ID (NCBI): 1048
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis. This antibody is raised against 35-685 aa of human CEA/CD66e and can cross-react with CEACAM1/CD66a and CEACAM3/CD66d.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jan (Verified Customer) (01-06-2025)

Immunofluorescence analysis of CEA in BxPC-3 cells. Cells were incubated with CEA/CD66e Recombinant antibody (83653-5-RR) and TRITC-conjugated Goat Anti-Rabbit IgG(H+L) (SA00007-2).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: BxPC-3

CEA/CD66e Antibody Immunofluorescence validation (1:250 dilution) in BxPC-3 (Cat no:83653-5-RR)