CEACAM1/CD66a Rekombinanter Antikörper

CEACAM1/CD66a Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

241856H5

Kat-Nr. : 84409-4-RR

Synonyme

241856H5, Bgp, Bgp1, BGP-1, Biliary glycoprotein 1

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  WB analysis using 84409-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84409-4-RR (CEACAM-1/CD66a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 84409-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84409-4-RR (CEACAM1/CD66a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 84409-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84409-4-RR (CEACAM1/CD66a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 84409-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84409-4-RR (CEACAM1/CD66a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 84409-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84409-4-RR (CEACAM1/CD66a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 84409-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CEACAM1/CD66a antibody (84409-4-RR, Clone: 241856H5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse small intestine using 84409-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using CEACAM1/CD66a antibody (84409-4-RR, Clone: 241856H5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84409-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84409-4-RR against Mouse CEACAM1/CD66a were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe, Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Dünndarmgewebe, Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84409-4-RR bindet in WB, IHC, IF-P, ELISA CEACAM1/CD66a und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CEACAM1/CD66a fusion protein Eg1423
• Vollständiger Name: carcinoembryonic antigen-related cell adhesion molecule 1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• Gene symbol: Ceacam1
• Gene ID (NCBI): 26365
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).