CEACAM1/CD66a Rekombinanter Antikörper

CEACAM1/CD66a Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241856H5

Kat-Nr. : 98185-1-RR

Synonyme

Biliary glycoprotein D, Biliary glycoprotein 1, BGP-1, Bgp1, Bgp

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  FC experiment of mouse splenocytes using 98185-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CEACAM1/CD66a Rabbit Recombinant Antibody (98185-1-RR, Clone: 241856H5) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD45R. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98185-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CEACAM1/CD66a Rabbit Recombinant Antibody (98185-1-RR, Clone: 241856H5) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD45R. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98185-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98185-1-RR against Mouse CEACAM1/CD66a were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98185-1-RR bindet in FC CEACAM1/CD66a und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: carcinoembryonic antigen-related cell adhesion molecule 1
• Berechnetes Molekulargewicht: 57 kDa
• Gene symbol: Ceacam1
• Gene ID (NCBI): 26365
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).