CEACAM1/CD66a Rekombinanter Antikörper

CEACAM1/CD66a Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240497B4

Kat-Nr. : 83524-3-RR

Synonyme

CEACAM1, Biliary glycoprotein 1, BGPI, BGP-1, BGP1

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  WB analysis using 83524-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83524-3-RR (CEACAM1/CD66a antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 83524-3-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83524-3-RR (CEACAM1/CD66a antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 83524-3-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83524-3-RR (CEACAM1/CD66a antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83524-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83524-3-RR against Human CEACAM1 were performed. The affinity constant is 3.58 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HT-29-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83524-3-RR bindet in WB, IHC, ELISA CEACAM1/CD66a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
• Berechnetes Molekulargewicht: 526 aa, 58 kDa
• Beobachtetes Molekulargewicht: 135-150 kDa
• GenBank-Zugangsnummer: BC014473
• Gene symbol: CEACAM1
• Gene ID (NCBI): 634
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).