CEACAM6/CD66c Rekombinanter Antikörper

CEACAM6/CD66c Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241997F7

Kat-Nr. : 84982-1-RR

Synonyme

CEACAM6, 241997F7, Carcinoembryonic antigen-related cell adhesion molecule 6, CD66c, CEAL

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  WB analysis using 84982-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84982-1-RR (CEACAM6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 84982-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84982-1-RR (CEACAM6/CD66c antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 84982-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84982-1-RR (CEACAM6/CD66c antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HT-29 using 84982-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CEACAM6/CD66c antibody (84982-1-RR, Clone: 241997F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84982-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84982-1-RR against Human CEACAM6 were performed. The affinity constant is 0.445 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HT-29-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84982-1-RR bindet in WB, IHC, IF/ICC, ELISA CEACAM6/CD66c und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CEACAM6/CD66c fusion protein Eg2450
• Vollständiger Name: carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen)
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 40-100 kDa
• GenBank-Zugangsnummer: BC005008
• Gene symbol: CEACAM6
• Gene ID (NCBI): 4680
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEACAM6, also known as CD66c, is a glycosylphosphatidylinositol (GPI)-linked cell surface protein that belongs to the CEACAM family of the immunoglobulin superfamily (PMID: 23903773). It is normally expressed on the surface of myeloid and epithelial surfaces and upregulated preferentially at the apical/luminal membranes of many tumors (PMID: 27595061; 35082925). CEACAM6 plays an important role in cell adhesion, proliferation, tumorigenesis, metastasis, and immunity (PMID: 31797958).