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CEACAM8/CD66b Rekombinanter Antikörper

CEACAM8/CD66b Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242954B2

Kat-Nr. : 83105-6-PBS

Synonyme

CD66b, CEACAM8, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, CD 66b

Filter:
  • Western Blot (WB) analysis of various lysates using CEACAM8/CD66b Recombinant antibody (83105-6-RR)

    WB analysis using 83105-6-RR (same clone as 83105-6-PBS)

    human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 83105-6-RR (CEACAM8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83105-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CEACAM8/CD66b Recombinant antibody (83105-6-RR)

    WB analysis using 83105-6-RR (same clone as 83105-6-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 83105-6-RR (CEACAM8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83105-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected CHO-K1 using CEACAM8/CD66b Recombinant antibody (83105-6-RR)

    IF Staining of Transfected CHO-K1 using 83105-6-RR (same clone as 83105-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Transfected CHO-K1 cells using CEACAM8 antibody (83105-6-RR, Clone: 242954B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83105-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83105-6-RR

    Affinity and Kinetic Characterization of 83105-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83105-6-RR against Human CEACAM8 were performed. The affinity constant is 14.1 nM.

Geprüfte Anwendungen

Produktinformation

83105-6-PBS bindet in WB, IF/ICC, Indirect ELISA CEACAM8/CD66b und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CEACAM8/CD66b fusion protein Eg32133
Vollständiger Name carcinoembryonic antigen-related cell adhesion molecule 8
Berechnetes Molekulargewicht 38 kDa
Beobachtetes Molekulargewicht95-100kDa
GenBank-ZugangsnummerBC026263
Gene symbol CEACAM8
Gene ID (NCBI) 1088
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8), also known as CD66b, CGM6 or NCA-95, is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAM8 is a glycosylphosphatidylinositol (GPI)-linked glycoprotein expressed on granulocytes (PMID: 8104998; 18056392). It is involved in regulating adhesion and activation of eosinophils (PMID: 18056392).