CEBP Alpha/CEBPA Rekombinanter Antikörper

CEBP Alpha/CEBPA Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242101C9

Kat-Nr. : 84668-4-RR

Synonyme

CEBP alpha, CEBPA, CEBP, CCAAT/enhancer-binding protein alpha, C/EBPα

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  IF Staining of HeLa using 84668-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CEBP Alpha/CEBPA antibody (84668-4-RR, Clone: 242101C9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 84668-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEBP Alpha/CEBPA antibody (84668-4-RR, Clone: 242101C9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 84668-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEBP Alpha/CEBPA antibody (84668-4-RR, Clone: 242101C9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 84668-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CEBP Alpha/CEBPA Recombinant antibody (84668-4-RR, Clone:242101C9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  FC experiment of HepG2 using 84668-4-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CEBP Alpha/CEBPA Recombinant antibody (84668-4-RR, Clone:242101C9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84668-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84668-4-RR against Human CEBP Alpha/CEBPA were performed. The affinity constant is 1.85 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:4000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84668-4-RR bindet in IF/ICC, FC (Intra), ELISA CEBP Alpha/CEBPA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CEBP Alpha/CEBPA fusion protein Ag29947
• Vollständiger Name: CCAAT/enhancer binding protein (C/EBP), alpha
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: NM_004364
• Gene symbol: CEBPA
• Gene ID (NCBI): 1050
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEBPA and its isoforms play important roles in lineage determination and gene activation in a variety of cell types by activating transcription from lineage-specific promoters. CEBPA is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. In hematopoiesis, C/EBPa is a key factor in driving the development of myeloid cells interacting with a variety of factors, including c-Myc, PU.1, and microRNAs. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin which plays an important role in body weight homeostasis. CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Several pathways have been implicated as the means by which CEBPA mediates cell cycle arrest and proliferation, including p21, cyclin-dependent kinases and the E2F complex via c-Myc.