CEBPB Monoklonaler Antikörper

CEBPB Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B6E10

Kat-Nr. : 66649-1-PBS

Synonyme

2B6E10, C EBP beta, C/EBP beta, C/EBP related protein 2, C/EBPbeta

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  WB analysis of HeLa using 66649-1-Ig (same clone as 66649-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66649-1-Ig (CEBPB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66649-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66649-1-Ig (same clone as 66649-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66649-1-Ig (CEBPB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66649-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66649-1-Ig (same clone as 66649-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66649-1-Ig (CEBPB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66649-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66649-1-Ig (same clone as 66649-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66649-1-Ig (CEBPB antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66649-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66649-1-PBS bindet in WB, IHC, Indirect ELISA CEBPB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CEBPB fusion protein Ag20073
• Vollständiger Name: CCAAT/enhancer binding protein (C/EBP), beta
• Berechnetes Molekulargewicht: 345 aa, 36 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC007538
• Gene symbol: CEBPB
• Gene ID (NCBI): 1051
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCAAT/enhancer-binding protein beta (CEBPB), also known as LAP, is a important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. It specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation By similarity. Regulates the transcriptional induction of peroxisome proliferator-activated receptor gamma (PPARG). CEBPb mRNAs possess alternative translation-initiation codons, which result in the formation of truncated forms of the protein. All major isoforms of CEBPB (38, 34, and 20 kDa) are expressed, with the 34 and 20kDa isoforms being more abundant in preovulatory follicles and further increased in corpora lutea (CL)(PMID:15647458).The truncated protein of 18 kDa (relative to the 30 kDa full-length protein that is known as LAP, or p30 CEBPb or liver-activating protein) lacks a transactivation domain,also known as LIP (p19 CEBPb or liver-inhibitory protein), can form homodimers or heterodimerize with other family members and, as it lacks the transactivation domain, can attenuate the transcriptional activation properties of the other isoforms.(10051447). Three variants of CEBPBs have been detected in many cell types: a 46 kDa full-length liver-enriched transcription-activating protein (LAP1), a 42 kDa LAP2 and a 20-kDa liver-enriched transcription-inhibitory protein (LIP). These variants are the result of an alternative translation initiation due to a leaky ribosomal scanning mechanism.(PMID:18820298).