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CEBPE Rekombinanter Antikörper

CEBPE Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251303B10

Kat-Nr. : 86432-1-PBS

Synonyme

C/EBP epsilon, CCAAT/enhancer-binding protein epsilon, CRP1

Filter:
  • Western Blot (WB) analysis of HL-60 cells using CEBPE Recombinant antibody (86432-1-RR)

    WB analysis of HL-60 using 86432-1-RR (same clone as 86432-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 86432-1-RR (CEBPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86432-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CEBPE Recombinant antibody (86432-1-RR)

    WB analysis of Jurkat using 86432-1-RR (same clone as 86432-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 86432-1-RR (CEBPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86432-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CEBPE Recombinant antibody (86432-1-RR)

    IF Staining of HepG2 using 86432-1-RR (same clone as 86432-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEBPE antibody (86432-1-RR, Clone: 251303B10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86432-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86432-1-PBS bindet in WB, IF/ICC, Indirect ELISA CEBPE und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CEBPE fusion protein Ag5589
Vollständiger Name CCAAT/enhancer binding protein (C/EBP), epsilon
Berechnetes Molekulargewicht 31 kDa
Beobachtetes Molekulargewicht 31-38 kDa
GenBank-ZugangsnummerBC035797
Gene symbol CEBPE
Gene ID (NCBI) 1053
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEBPE (CCAAT/enhancer binding protein epsilon), also known as C/EBP-epsilon and CRP1, is a transcription factor involved in late myeloid lineage differentiation and cellular function (PMID: 31201888). CEBPE is expressed in a stage-specific manner during myeloid differentiation and regulates transition from the promyelocyte to the myelocyte (PMID: 29773596). Depletion of CEBPE in Acute lymphoblastic leukaemia (ALL) cells reduces cell growth, correspondingly CEBPE binds to the promoters of electron transport and energy generation genes (PMID: 29977016).