CEBPE Rekombinanter Antikörper

CEBPE Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251303B10

Kat-Nr. : 86432-1-RR

Synonyme

C/EBP epsilon, CCAAT/enhancer-binding protein epsilon, CRP1

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  WB analysis of HL-60 using 86432-1-RR

  HL-60 cells were subjected to SDS PAGE followed by western blot with 86432-1-RR (CEBPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 86432-1-RR

  Jurkat cells were subjected to SDS PAGE followed by western blot with 86432-1-RR (CEBPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 86432-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEBPE antibody (86432-1-RR, Clone: 251303B10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HL-60-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86432-1-RR bindet in WB, IF/ICC, ELISA CEBPE und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CEBPE fusion protein Ag5589
• Vollständiger Name: CCAAT/enhancer binding protein (C/EBP), epsilon
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 31-38 kDa
• GenBank-Zugangsnummer: BC035797
• Gene symbol: CEBPE
• Gene ID (NCBI): 1053
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEBPE (CCAAT/enhancer binding protein epsilon), also known as C/EBP-epsilon and CRP1, is a transcription factor involved in late myeloid lineage differentiation and cellular function (PMID: 31201888). CEBPE is expressed in a stage-specific manner during myeloid differentiation and regulates transition from the promyelocyte to the myelocyte (PMID: 29773596). Depletion of CEBPE in Acute lymphoblastic leukaemia (ALL) cells reduces cell growth, correspondingly CEBPE binds to the promoters of electron transport and energy generation genes (PMID: 29977016).