CEBPG Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CEBPG Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 12997-1-AP

Synonyme

IG/EBP 1, GPE1BP, CCAAT/enhancer-binding protein gamma, C/EBP gamma

---

• 

  IHC staining of mouse liver using 12997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12997-1-AP (CEBPG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 12997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12997-1-AP (CEBPG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 12997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12997-1-AP (CEBPG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 12997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12997-1-AP (CEBPG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 12997-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12997-1-AP (CEBPG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 12997-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12997-1-AP (CEBPG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 12997-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CEBPG antibody (12997-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Mauslebergewebe, Maus-Kolongewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

12997-1-AP bindet in WB, IHC, IF/ICC, ELISA CEBPG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEBPG fusion protein Ag3663
• Vollständiger Name: CCAAT/enhancer binding protein (C/EBP), gamma
• Berechnetes Molekulargewicht: 150 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16-18 kDa
• GenBank-Zugangsnummer: BC013128
• Gene symbol: CEBPG
• Gene ID (NCBI): 1054
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IHC	Neuron; Core transcription programs controlling injury-induced neurodegeneration of retinal ganglion cells.; Authors - Feng Tian ; KD Validated
human	WB	Int J Biol Sci; Novel LncRNA LINC02936 Suppresses Ferroptosis and Promotes Tumor Progression by Interacting with SIX1/CP Axis in Endometrial Cancer; Authors - Zihui Zhang
human	WB,IHC	Am J Cancer Res; CEBPG promotes esophageal squamous cell carcinoma progression by enhancing PI3K-AKT signaling.; Authors - Yongsheng Huang ; KD Validated
human	IHC	Cancer Inform; Comprehensive Analysis of CCAAT/Enhancer Binding Protein Family in Ovarian Cancer; Authors - Jiahong Tan
human	IF	Cell Biosci; Single-cell multi-omics analysis reveals candidate therapeutic drugs and key transcription factor specifically for the mesenchymal subtype of glioblastoma; Authors - Yufan Yang
human,mouse	WB	Autophagy; RETREG1-mediated reticulophagy is activated by an ATF4-CEBPG/C/EBPγ heterodimer and confers protection against lipotoxicity; Authors - Suwei Jin